S-Glutathionylated Protein Detection Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • assays kits measures measurements flow cytometry cytometer fluorescence microscopy GSH tripeptides gamma-glutamyl g-glutamyl .gamma.-glutamyl ?-glutamylcsteinylglyceines proteins sufhydryls reduction plasma tissues livers disulfides cells metabolic structural cytoskeletal signaling adduct antibodies Biotin-Switch method whole permeabilized avidin overlay analysis free-thiols enzymatic cleavages protein-S-glutathiones streptavidin-based glutathione
• Product Overview:
  Glutathione (GSH) is a tripeptide (?-glutamylcysteinylglycine) widely distributed in both plants and animals.{1813,4672} GSH is involved in maintenance of protein sulfhydryl reduction status.{4669,4670} The concentration of GSH ranges from a few micromolar in plasma to several millimolar in tissues such as liver.{4671,4673} Mixed protein glutathionyl disulfides are a post translational protein modification of growing interest.{11632,17080,15065} Protein S-glutathionylation (PSSG) may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins.{16795} PSSG detection methods can employ GSH adduct antibodies, GSH derivatives, and differential labeling systems based on the “Biotin-Switch” method.{15065,13441,12802} Cayman’s S-Glutathionylated Protein Detection Assay Kit provides a convenient method for the direct visualization of S-glutathionylated proteins in whole (permeabilized) cells by flow cytometry and microscopy as well as avidin overlay analysis.{15066} This cell-based assay starts with the modification of protein free-thiols groups followed by enzymatic cleavage of any protein-S-glutathione (PSSG) adducts present in the sample. Biotinylation of the newly-formed protein free-thiols provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Reagents are provided to test three sets of 10 samples (most convenient) or up to thirty samples total at once if desired.