€873.00 €742.05
Protein-arginine N-methyltransferase 5 (PRMT5) is a type II PRMT and member of the class I S-adenosylmethionine-dependent methyltransferases that symmetrically dimethylates arginine residues in proteins and histones.{63086} It is composed of an N-terminal TIM domain and a C-terminal catalytic domain. PRMT5 proteins interact with methylosome 50 (MEP50) proteins primarily via the TIM domain to form a hetero-octameric complex that is more active than PRMT5 alone.{63087} Both PRMT5 and MEP50 are found in a wide variety of somatic and embryonic tissues, including the fetal ovary, fetal heart, and adult retina.{63088} The PRMT5-MEP50 complex methylates spliceosomal proteins and newly synthesized histone H2A in the cytoplasm and interacts with additional binding partners to regulate transcription through the methylation of NF-?B and p53 and other transcription factors in the nucleus. PRMT5 and MEP50 expression is increased in a variety of cancers and correlated with lower survival rates in patients with non-small cell lung cancer (NSCLC). Mutations in each gene are also associated with many types of cancer. Cayman’s PRMT5-MEP50 protein can be used for enzyme activity assays.
Territorial Availability: Available through Bertin Technologies only in France
| Size | 20 µg |
|---|---|
| Shipping | dry ice |
| Molecular weight | 0 |
| Formulation | 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 80 µg/ml DYKDDDDK peptide, and 20% glycerol |
| Purity | ≥89% estimated by SDS-PAGE |
| Custom code | 3504.00 |
| UNSPSC code | 12352203 |
Cayman Chemical’s mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.
Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.
Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.
Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.
Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009
Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.
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