Antioxidant Assay Kit

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Cat No: 709001

Assay Kits - Colorimetric Assays

Cayman’s Antioxidant Assay can be used to measure the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. Aqueous- and lipid-soluble antioxidants are not separated in this protocol, thus the combined antioxidant activities of ...

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Territorial Availability: Available through Bertin Technologies only in France

Product Overview:
Cayman’s Antioxidant Assay can be used to measure the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. Aqueous- and lipid-soluble antioxidants are not separated in this protocol, thus the combined antioxidant activities of all its constituents including vitamins, proteins, lipids, glutathione, uric acid, etc. are assessed. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS® (2,2’-Azino-di-[3-ethylbenzthiazoline sulphonate]) to ABTS®•+ by metmyoglobin. The amount of ABTS®•+ produced can be monitored by reading the absorbance at 750 nm or 405 nm. Under the reaction conditions used, the antioxidants in the sample cause suppression of the absorbance at 750 nm or 405 nm to a degree which is proportional to their concentration.2-5 The capacity of the antioxidants in the sample to prevent ABTS® oxidation is compared with that of Trolox, a water-soluble tocopherol analogue, and is quantified as millimolar Trolox equivalents.

Size	96 wells
Shipping	wet ice
Custom Code	3822.19
UNSPSC code	41116104

Miller, N.J., Rice-Evans, C., Davies, M.J., et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84 407-412 (1993).

Miller, N.J., and Rice-Evans, C. F

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.