Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Size | 96 wells |
Shipping | Dry ice |
Specificity | |
Application Media | Plasma, serum and culture supernatant|No extraction required |
Sample volume | 50 µL |
Tracer | AcetylCholinesterase AChE |
Detection Limit | 0,2 ng/mL |
Standard Curve Range | 0,4-50 ng/mL |
Custom Code | 3822000000 |
UNSPSC code | 41116104 |
PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS
Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991
Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)
Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34
PRODUCT EXPLANATION : PROLACTIN
Freeman M.E, Kanyicska B., Lerant A. et al. Prolactin: Structure, Function, and Regulation of Secretion. Physiological Reviews, Vol. 80, No. 4, October 2000
PRODUCT EXPLANATION: KIT DEVELOPMENT
Duhau L, Grassi J, Grouselle D; et al. An Enzyme Immunoassay for rat Prolactin: application to the determination of plasma levels. J. Immunoassay, 12(2), 233-250, 1991
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011
PRODUCT CITATIONS
Nikishina Y.O., Sapronova A.Y., Ugrumov M.V. The Effect of Dopamine Secreted by the Brain into the Systemic Circulation on Prolactin Synthesis by the Pituitary gland in Ontogenesis. Acta Naturae, vol. 8 № 3 (30) 2016
Suzuki Y., Nakahara K., Maruyama K. et al. Changes in mRNA expression of arcuate nucleus appetite-regulating peptides during lactation in rats. Journal of Molecular Endocrinology; April 1, 2014; vol.52; 97-109
Capasso R. Effect of Silitidil, a Standardized Extract of Milk Thistle, on the Serum Prolactin Levels in Female Rats. Natural Product Communications Vol. 9 (7) 2014
Kanisra Mary F., Eswaramohan T., Surendran S.N. et al. Mukkuddu Maathrai (Triple Tablet), An Indigenous Medicine Elevates Serum Prolactin Level Of Female Rats (Rattus norvegicus). IOSR Journal Of Pharmacy, Volume 3, Issue (January 2013), PP.38-42
Calejo A. I., Jorgacevski J., Silva V.S. et al. Aluminium-induced changes of fusion pore properties attenuate prolactin secretion in rat pituitary lactotrophs. Neuroscience 201 (2012), 57-66
Pujianto D.A, Curry B.J. and Aitken R.J. Prolactin Exerts a Prosurvival Effect on Human Spermatozoa via Mechanisms that Involve the Stimulation of Akt Phosphorylation and Suppression of Caspase Activation and Capacitation. Endocrinology, March 2010, 151(3):1269–1279
Miyoshi T., Otsuka F., Otani H. et al. Involvement of bone morphogenetic protein-4 in GH regulation by octreotide and bromocriptine in rat pituitary GH3 cells. Journal of Endocrinology (2008) 197, 159-169