Akt1 (human, recombinant)

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Cat No: 10011209

Proteins - Enzymes

Akt1, also known as protein kinase Bα (PKBα), is a serine/threonine kinase belonging to the AGC kinase family and one of three Akt isoforms in mammals.{57326,59549} Akt kinases function downstream of activated tyrosine kinases and PI3K to regulate a v...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

Akt PIP PKB RAC? RAC-PK? RACPK? RACPK-?

Product Overview:
Akt1, also known as protein kinase Bα (PKBα), is a serine/threonine kinase belonging to the AGC kinase family and one of three Akt isoforms in mammals.{57326,59549} Akt kinases function downstream of activated tyrosine kinases and PI3K to regulate a variety of cellular processes, including cell size, growth, proliferation, and survival, as well as genome stability, glucose metabolism, and neovascularization.{59549} Akt1, like Akt2 and Akt3, is composed of an N-terminal pleckstrin homology (PH) domain, which binds to phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and phosphatidylinositol-(3,4)-diphosphate (PIP2), a kinase domain, and a C-terminal regulatory hydrophobic motif. It is ubiquitously expressed and is the primary isoform in endothelial cells.{15560} Akt1 is activated via recruitment to the plasma membrane, which is mediated by the interaction of the Akt1 PH domain with PI3K-generated PIP3 and PIP2, and subsequent phosphorylation at threonine 308 and serine 473.{59549} Increased Akt1 kinase activity has been found in tumor tissue isolated from patients with prostate, breast, and ovarian cancers.{59550} Cayman’s Akt1 (human, recombinant) protein was phosphorylated by recombinant 3-phosphoinositide-dependent kinase 1 (PDK1) and can be used for enzyme activity assays.

Size	25 µg
Shipping	dry ice
Formulation	50 mM HEPES, pH 8.0, with 150 mM sodium chloride, 1 mM DTT, and 10% glycerol
Purity	≥95% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.