SureLight™ Glow - Firefly Lucife

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SureLight™ Glow - Firefly Luciferase Cell Viability Assay

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Cat No: 34972

Assay Kits - More Assay Kits

The SureLight™ Glow firefly luciferase cell viability assay provides a homogeneous and highly sensitive method for quantifying ATP, an indicator of cell viability, in cell cultures. The assay generates a stable, glow-type luminescent signal directly p...

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Territorial Availability: Available through Bertin Technologies only in France

Product Overview:
The SureLight™ Glow firefly luciferase cell viability assay provides a homogeneous and highly sensitive method for quantifying ATP, an indicator of cell viability, in cell cultures. The assay generates a stable, glow-type luminescent signal directly proportional to the amount of viable cells present in the cell culture. This kit includes: Firefly Luciferase (lyophilized) D-Luciferin (lyophilized) Lysis Buffer (frozen) Material to be supplied by user: Opaque-walled multi-well plate Multichannel pipette Plate shaker Luminometer or imaging device capable of reading multi-well plates Reagent Preparation: 1. Thaw buffer; equilibrate to room temperature 2. Reconstitute lyophilized enzyme/substrate with buffer 3. Mix by vortexing or gently inverting Protocol for Assay: 1. Prepare opaque-walled multi-well plates with mammalian cells in culture medium, (e.g.,100 µl per well for 96-well plates) 2. Prepare control wells containing medium without cells 3. Add the test compound to experimental wells and incubate according to the cell culture protocol 4. Equilibrate the plate and its contents at room temperature, approximately 15-30 minutes 5. Add a volume of cell viability reagent equal to the volume of medium containing cells in each well (e.g., 100 ul of reagent to 100 ul of cell culture medium) 6. Mix contents on an orbital shaker to induce cell-lysis 7. Allow the plate to incubate at room temperature for 5-15 minutes to stabilize luminescent signal 8. Read results on luminometer

Size	100 tests
Shipping	dry ice
Application(s)	Cell viability, HTS
Custom Code	3822.19
UNSPSC code	41116104

Technical File

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.