12-Lipoxygenase (leukocyte-type;

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12-Lipoxygenase (leukocyte-type; mouse) Polyclonal Antibody

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Cat No: 32822

12-Lipoxygenase (12-LO) is one of several LOs that catalyzes the peroxidation of polyunsaturated fatty acids in a position-specific manner.{59716} There are four 12-LO subtypes, encoded by distinct genes, that can be categorized according to their tis...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

12/15LO 12LO L12LO 15LO Alox 15 L-12-LO Arachidonate Omega-6 Lipoxygenase Hepoxilin A3 Synthase Linoleate 13S-lipoxygenase PUFA poly peptide IL4 IL13 LNAME

Product Overview:
12-Lipoxygenase (12-LO) is one of several LOs that catalyzes the peroxidation of polyunsaturated fatty acids in a position-specific manner.{59716} There are four 12-LO subtypes, encoded by distinct genes, that can be categorized according to their tissue expression in mice.{59714} Leukocyte-type 12-LO is encoded by Alox15 in mice and shares 74% sequence identity with human ALOX15 but, in contrast to its human ortholog, encodes a 12-lipoxygenating ALOX15 isoform.{54602,59715,59713} Leukocyte-type 12-LO exists as a single polypeptide chain composed of an N-terminal regulatory domain and a C-terminal catalytic domain.{59716} It catalyzes the oxidation of arachidonic acid (Item Nos. 90010
90010.1
10006607) to 12(S)-HpETE (Item No. 44570) and, to a lesser extent, 15(S)-HpETE (Item No. 44720), which are further reduced to 12(S)-HETE (Item No. 34570) and 15(S)-HETE (Item No. 34720), respectively.{59716,33744,3606} Other substrates of leukocyte-type 12-LO include eicosapentaenoic acid, docosahexaenoic acid, linoleic acid, and γ-linolenic acid, which are oxygenated at various positions in a substrate-dependent manner.{59717} Leukocyte-type 12-LO is constitutively expressed at high levels in resident peritoneal macrophages, and its activity is increased by the Th2 cytokines IL-4 and IL-13 in vitro.{59713} Alox15-/- mice are protected from increases in systolic blood pressure in a model of hypertension induced by L-NAME (Item No. 80210).{59718} Cayman's 12-Lipoxygenase (leukocyte-type; mouse) Polyclonal Antibody can be used for ELISA and Western blot (WB) applications. The antibody recognizes 12-LO (leukocyte-type) at ~73 kDa from porcine and mouse samples.

Size	500 µg
Shipping	dry ice
Host	Rabbit
Antigen	Full-length recombinant mouse 12-lipoxygenase (leukocyte-type)
Application(s)	ELISA and WB
Formulation	500 µg of protein A-purified polyclonal antibody
Custom Code	3822.19
UNSPSC code	12352203

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.