Cyclin B1 Rabbit Monoclonal Anti

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Cyclin B1 Rabbit Monoclonal Antibody

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Cat No: 32231

Cyclin B1 is a regulatory protein responsible for the initiation of mitosis.{59642} It contains an unstructured N-terminal domain that associates with mitotic chromosomes, a D-box sequence, which regulates the degradation of cyclin B1, a cytoplasmic r...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

Dbox Cdk G 2 Plk CCNB D over-expressed p 53 si RNA

Product Overview:
Cyclin B1 is a regulatory protein responsible for the initiation of mitosis.{59642} It contains an unstructured N-terminal domain that associates with mitotic chromosomes, a D-box sequence, which regulates the degradation of cyclin B1, a cytoplasmic retention sequence (CRS), and a cyclin box domain, which binds cyclin-dependent kinase 1 (Cdk1) and contains an MRAIL motif that associates with centrosomes.{59643} Cyclin B1 forms a complex with Cdk1 and is localized primarily to the cytoplasm until cyclin B1 protein levels peak at the G2/M transition.{59642} Cyclin B1 is then phosphorylated at the CRS by Cdk1 and polo-like kinase 1 (Plk1), which triggers the translocation of the complex to the nucleus where it phosphorylates a variety of substrates to initiate mitosis. When cyclin B1 is ubiquitinated and degraded, the cell exits anaphase. CCNB1, the gene encoding cyclin D1, is overexpressed throughout the cell cycle in cancer cells lacking the tumor suppressor p53 and acts as a tumor antigen.{59644,59645} CCNB1 overexpression in an esophageal squamous cell carcinoma (ESCC) mouse xenograft model increases invasion and metastasis, while siRNA suppression of cyclin B1 expression inhibits metastasis to the lungs.{59646} In contrast, overexpression of CCNB1 in patient-derived colorectal cancer tissue negatively correlates to metastasis and low expression positively correlates to poorer overall survival.{59647} Cayman’s Cyclin B1 Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

Size	100 µl
Shipping	dry ice
Host	Rabbit
Antigen	Peptide corresponding to cyclin B1
Clone	RM281
Isotype	IgG
Application(s)	IHC, WB
Formulation	100 µl of protein A-affinity purified monoclonal antibody
Custom Code	3822.19
UNSPSC code	12352203

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.