CISD1 Cytosolic Domain (human, r

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CISD1 Cytosolic Domain (human, recombinant)

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Cat No: 32034

Proteins - More Proteins

CDGSH iron sulfur domain 1 (CISD1), also known as mitoNEET, is an iron-sulfur outer mitochondrial membrane protein and member of the NEET protein family with roles in redox sensing, iron uptake, and respiratory capacity.{53739,53740} It is a homodimer...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

Escherichia CISD-1 mito-neet 2Fe2S Hep-G2 HL1 Zinc Finger CDGSH-Type Domain 1 Iron-Sulfur Domain-Containing Protein 1 Chromosome 10 Open Reading Frame 70 ZCD-1 MDS029

Product Overview:
CDGSH iron sulfur domain 1 (CISD1), also known as mitoNEET, is an iron-sulfur outer mitochondrial membrane protein and member of the NEET protein family with roles in redox sensing, iron uptake, and respiratory capacity.{53739,53740} It is a homodimeric protein comprised of a β-cap domain and a cluster binding domain with each monomer consisting of an N-terminal in-organelle domain, a transmembrane domain, and a C-terminal cytosolic domain.{53739} The CISD1 C-terminal domain houses the [2Fe-2S] cluster binding site which has roles in tuning redox properties of the protein in response to environmental conditions. CISD1 mRNA expression is induced by the ferroptosis inducer erastin (Item No. 17754) in HepG2 cells in an iron-dependent manner and stabilization of the CISD1 [2Fe-2S] cluster by pioglitazone (Item Nos. 71745
22263
10028) inhibits mitochondrial iron uptake, lipid peroxidation, and subsequent ferroptosis in the same cells.{53740} Overexpression of CISD1 decreases oxidative stress-induced apoptosis in an HL-1 cardiomyocyte model of hypoxia and reoxygenation injury.{53738} Levels of CISD1 mRNA are decreased in subcutaneous and visceral adipose tissue isolated from patients with obesity and visceral adipose tissue levels of CISD1 mRNA are positively correlated with insulin sensitivity in patients with morbid obesity.{55227} Knockdown of Cisd1 in mice induces mitochondrial dysfunction and increased levels of mitochondrial reactive oxygen species (ROS), as well as a loss of striatal dopamine, shortened stride length in a gait analysis, and decreased latency to fall in the rotarod test, all of which serve as markers of a Parkinsonian phenotype.{53737} Cayman's CISD1 Cytosolic Domain (human, recombinant) protein consists of 95 amino acids and has a calculated molecular weight of 11.3 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass is approximately 14 kDa.

Size	100 µg
Shipping	dry ice
Formulation	Lyophilized from sterile 20 mM Tris, with 150 mM sodium chloride and 10% glycerol
Purity	≥90% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

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ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.