TNFRSF8/CD30 Long Isoform Extrac

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TNFRSF8/CD30 Long Isoform Extracellular Domain (human, recombinant)

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Cat No: 31829

Proteins - More Proteins

CD30, also known as tumor necrosis factor receptor superfamily member 8 (TNFRSF8), is a type I transmembrane glycoprotein encoded by TNFRSF8 in humans.{52838,52839} Alternative splicing of the TNFRSF8 pre-mRNA produces one full-length isoform, CD30 lo...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

CD-30 TNFRSF-8 30L TRAF-1 2 5 NF?B NF?-B IgG Phe-19 HEK-293 Ki1 D1S166-E D-1S166E Jun-B

Product Overview:
CD30, also known as tumor necrosis factor receptor superfamily member 8 (TNFRSF8), is a type I transmembrane glycoprotein encoded by TNFRSF8 in humans.{52838,52839} Alternative splicing of the TNFRSF8 pre-mRNA produces one full-length isoform, CD30 long, and two short isoforms that contain truncated transmembrane and/or extracellular domains.{52838,52840} CD30 long exists as a transmembrane monomer and is composed of an extracellular domain containing three cysteine-rich repeats (CRDs), a transmembrane domain, and a cytoplasmic domain that facilitates intracellular signaling.{52839} It is expressed on certain subsets of activated T- or B cells, as well as in a variety of lymphoid neoplasms, including Hodgkin lymphoma and anaplastic large cell lymphomas (ALCLs).{52838} It can also exist in a soluble form, which is produced by proteolytic cleavage of CD30 at the cell surface and is found in the serum.{52838} CD30 is upregulated by mitogens, such as phorbol 12-myristate 13-acetate (TPA; Item No. 10008014), or viral stimulation, including Epstein-Barr virus (EBV) or HIV, as well as by the transcription factor JunB.{52840,52838} Upon interaction with its ligand, TNFSF8/CD30L, which is also expressed on certain subsets of activated lymphocytes, CD30 assembles into a trimer that recruits TNF receptor-associated factor 1 (TRAF1), TRAF2, and TRAF5 to induce NF-κB- or ERK/MAPK-dependent downstream signaling, mediating a variety of cellular processes, including NF-κB activation, cell proliferation and survival, as well as apoptosis.{52838,52839} Neutralization of CD30 with a monoclonal antibody reduces tumor growth in an ALCL patient-derived xenograft (PDX) mouse model.{52841} Serum levels of soluble CD30 (sCD30) are correlated with disease stage in patients with Hodgkin lymphoma or systemic lupus erythematosus (SLE).{52839} Cayman's TNFRSF8/CD30 Long Isoform (human, recombinant) protein can be used for binding assay applications. This protein is a disulfide-linked homodimer. The reduced monomer, comprised of CD30 (amino acids 19-379) fused to His-tagged human IgG1 Fc at its C-terminus, consists of 609 amino acids, has a calculated molecular weight of 66.5 kDa, and a predicted N-terminus of Phe19 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 130 kDa by SDS-PAGE under reducing conditions.

Size	100 µg
Shipping	dry ice
Formulation	Lyophilized from sterile PBS, pH 7.4
Purity	≥90% estimated by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.