SARS-CoV-2 nsp16 Methyltransfera

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SARS-CoV-2 nsp16 Methyltransferase

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Cat No: 31816

Proteins - Enzymes

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-...

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Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

Escherichia SARS-CoV2 SARSCoV2 SARSCoV COVID19 nsp-16 Cap1 SCoV2-sp16

Product Overview:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.{57176} SARS-CoV-2 nsp16 methyltransferase is a 2'-O-methyltransferase (2'-O-MTase) and has a role in 5'-end capping of viral mRNAs.{52793,52794} In coronaviruses, nsp16 forms a complex with nsp10 to methylate nascent mRNAs at the ribose 2'-O position, creating a Cap-1 structure that facilitates increased translation of viral mRNAs and reduced innate immune recognition by the host cell.{52794,52795} Deficiency of nsp16 in the related virus SARS-CoV reduces viral RNA synthesis by approximately 10-fold in vitro, and mutations in the nsp16 KDKE catalytic tetrad in mouse-adapted SARS-CoV attenuate the virus in vivo.{52794,52796} An nsp10-derived peptide inhibitor of the nsp16/nsp10 complex increases survival in a mouse model of infection with the coronavirus mouse hepatitis virus (MHV).{52795} Cayman's SARS-CoV-2 nsp16 Methyltransferase protein consists of 299 amino acids and has a calculated molecular weight of 33.46 kDa.

Size	100 µg
Shipping	dry ice
Formulation	Lyophilized from sterile 20mM Tris 150mM sodium chloride, pH 8.3, with 5% trehalose, 5% mannitol, and 0.01% tween-80
Purity	≥85% as determined by SDS-PAGE
Custom Code	3504.00
UNSPSC code	12352204

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Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.