Dopamine Transporter (Phospho-Th

Confirm delete?

Dopamine Transporter (Phospho-Thr⁵³) Polyclonal Antibody

Zoom

Cat No: 29261

The dopamine transporter (DAT) is a member of the SLC6 family of transporters and is encoded by the SLC6A3 gene in humans.{55189,55190} DAT is expressed in dopaminergic neurons and localizes to perisynaptic sites, where it functions to translocate the...

This product can only be bought through Cayman Chemical. Please contact us.

Product Overview
Data
Downloads
About Cayman

Territorial Availability: Available through Bertin Technologies only in France

Correlated keywords:

phosphoThr53 phosphoThr Thr 53 SLC 6 A3 post translational LLCPK1 PK 1 nonphosphorylatable phospho mimetic 3H MPP DAT 1 DAT1 PKDYS Sodium dependent Solute carrier family 6 Variable number tandem repeat VNTR

Product Overview:
The dopamine transporter (DAT) is a member of the SLC6 family of transporters and is encoded by the SLC6A3 gene in humans.{55189,55190} DAT is expressed in dopaminergic neurons and localizes to perisynaptic sites, where it functions to translocate the neurotransmitter dopamine (DA; Item No. 21992) into presynaptic neurons from the extracellular space.{55191,55189} It is comprised of 12 transmembrane helices flanked by large cytoplasmic N- and C-terminal tails that are subject to protein-protein interactions with DAT binding proteins as well as posttranslational modifications.{55189,55191} DAT can be phosphorylated by ERK at threonine 53 (Thr53) in vitro.{55192} Phosphorylation of DAT at Thr53 (phospho-Thr53) is increased in rat striatal synaptosomes in response to phorbol 12-myristate 13-acetate (PMA; Item No. 10008014), okadaic acid (Item No. 10011490), amphetamine, or methamphetamine.{55192,55193} In vivo, phospho-Thr53 in rat striatum is increased following administration of methamphetamine.{55193} LLC-PK1 cells expressing rat DAT (rDAT) with non-phosphorylatable or phosphomimetic Thr53 mutations exhibit defects in DA uptake and amphetamine-induced efflux of the substrate [3H]MPP+ compared to cells expressing wild-type rDAT, indicating that phosphorylation of Thr53 has roles in regulating the uptake and efflux functions of DAT.{55192} Cayman's Dopamine Transporter (Phospho-Thr53) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes DAT (phospho-Thr53) at approximately 55 kDa from mouse and rat samples.

Size	100 µl
Shipping	dry ice
Host	Rabbit
Antigen	Phosphopeptide corresponding to amino acid residues surrounding phospho-Thr53 of rat DAT
Application(s)	WB
Formulation	100 µl of affinity-purified polyclonal antibody from pooled serum
Custom Code	3822.19
UNSPSC code	12352203

Technical File

MSDS

Click here to ask for your quote and get 15% off Cayman's products.

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.

Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009

Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.