€744.00 €632.40
Peroxisome Proliferator Activated Receptors (PPARs) are ligand-activated transcription factors belonging to the large superfamily of nuclear receptors.{7660,12196} They are activated by a variety of fatty acids and fatty acid derivatives such as prostaglandins and leukotrienes. PPARs play pivotal roles in the regulation of lipid metabolism and homeostasis and are important indirect as well as direct regulators of cellular insulin sensitivity.{10561} There are three major PPAR isotypes; PPAR?, PPAR?, and PPAR?/? which all bind to PPAR responsive elements (PPRE’s) as heterodimers with RXR, another member of the nuclear receptor superfamily. PPAR? primarily activates genes encoding proteins involved in fatty acid oxidation, while PPAR? primarily activates genes directly involved in lipogenic pathway and insulin signaling.{7660,8987,10011} Members of the PPAR family are important direct targets of many antidiabetic and hypolipidemic drugs.{6047} Cayman’s PPAR?, ?, ? Complete Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the PPAR response element is immobilized onto the bottom of wells of a 96 well plate. PPARs contained in a nuclear extract, bind specifically to the PPAR response element. PPAR?, ?, or ? are detected by addition of specific primary antibodies directed against the individual PPARs. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman’s PPAR?, ?, ? Complete Transcription Factor Assay comes with a single plate that measures all three isoforms of PPAR?, ?, and ?. There are enough reagents for one-third of a plate for each isoform.
Territorial Availability: Available through Bertin Technologies only in France
| Size | 96 wells |
|---|---|
| Shipping | dry ice |
| Molecular weight | 0 |
| Custom code | 3822.19 |
| UNSPSC code | 41116104 |
Cayman Chemical’s mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.
Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.
Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.
Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.
Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009
Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.
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