€542.00 €460.70
3-Phosphoinositide-dependent protein kinase 1 (PDK1) is a serine/threonine kinase with roles in cell survival, differentiation, and proliferation, metabolic regulation, and immune development.{65465,65466,65467} It is composed of an N-terminal bilobular kinase domain, with the small lobe housing a PIF-pocket/?C-helix region and the large lobe containing an activation loop, and a C-terminal PH domain.{65465} PDK1 is activated by autophosphorylation of serine 241 in the activation loop, a residue that is poorly accessible to phosphatases, and is considered constitutively active.{65465} It phosphorylates and activates various members of the AGC protein kinase family, including Akt, p70 ribosomal S6 kinase (p70S6K), serum/glucocorticoid regulated kinase (SGK), and PKC to regulate the PI3K/Akt, Ras/MAPK, and Myc signaling pathways.{65465,65466} Gene amplification of PDPK1, the gene encoding PDK1, is associated with poor prognosis in patients with breast cancer and metastasis in patients with prostate cancer.{65465} Knockdown of Pdpk1 promotes axon regeneration in a mouse model of sciatic nerve injury and inhibits T follicular helper (Tfh) cell differentiation and germinal center responses in a mouse model of acute lymphocytic choriomeningitis virus (LCMV) infection.{65466,65467} Cayman’s PDK1 (human, recombinant) protein is expressed with an N-terminal His-TEV tag, which is removed during the protein purification process, leaving behind an N-terminal glycine residue on the full-length protein. This protein can be used for the phosphorylation of other proteins, such as Akt, and Western blot (WB).
Territorial Availability: Available through Bertin Technologies only in France
| Size | 10 µg |
|---|---|
| Shipping | dry ice |
| Molecular weight | 0 |
| Formulation | 50 mM Tris-HCl, pH 7.4, with 150 mM sodium chloride and 10% glycerol |
| Purity | ≥85% estimated by SDS-PAGE |
| Custom code | 3504.00 |
| UNSPSC code | 12352204 |
Cayman Chemical’s mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.
Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.
Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.
Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.
Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009
Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.
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