OX40/CD134 (human, recombinant)

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • OX 40 40L CD 134 TNF RSF4 TRAF 1 super-family intra-cellular auto-immune Leu 29 HEK 293 ACT 35 IMD 16 TXGP 1L
• Product Overview:
  OX40, also known as TNF receptor superfamily member 4 (TNFRSF4), is a type I transmembrane glycoprotein and the cognate receptor for the T cell co-stimulatory molecule OX40L (TNFSF4; Item No. 32065).{59402} OX40 assembles into trimers, with each monomer containing three cysteine-rich domains (CRDs) that bind OX40L and a cytoplasmic tail that interacts with TNF receptor-associated factor 1 (TRAF1), -2, -3, and -5 to induce a variety of intracellular signaling cascades, including NF-κB, ERK, PI3K, and Akt pathways.{59402,52886} OX40 is transiently expressed on CD4+ and CD8+ T cells in response to antigen stimulation in humans and is localized to lipid rafts.{59402,57029} Binding of an OX40 trimer to trimeric OX40L, expressed by antigen-presenting cells (APCs), including dendritic cells (DCs), macrophages, and B cells, forms a hexameric OX40-OX40L complex that promotes effector T cell phenotype and function, proliferation, survival, and memory.{57029} Increased OX40 levels have been found in inflamed tissues isolated from patients with a variety of autoimmune diseases, including multiple sclerosis, systemic lupus erythematosus (SLE), or rheumatoid arthritis.{57029} Cayman’s OX40/CD134 (human, recombinant) protein can be used for binding assays. This protein is a disulfide-linked homodimer. The reduced monomer, composed of OX40 (amino acids 29-216) fused to His-tagged human IgG1 Fc at its C-terminus, consists of 436 amino acids, has a calculated molecular weight of 48.2 kDa, and a predicted N-terminus of Leu29 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 68 kDa by SDS-PAGE under reducing conditions.