Orexin 1 Receptor Reporter Assay Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • hypercretins 1 orexins 2 A orexin-1 systems regulates regulator sleep wake cycles feeding cardiovascular autonomic ANS hypothalamic neuropeptides B gut hormones secretions hypothalamus midbrains brainstems G protein-coupled receptors GPCRs orexin-1 OX1R orexin-2 OX2R orexin-A orexin-B disorders narcolepsy humans animals rodents energy homeostasis pain stress antagonists drugs addictions withdrawals therapeutics obesity Surface transfection expression protocols solid phase transient transfection technology originus patents USPT US6897067 US6902933 US7056741 US 6897067 6902933 7056741 DNA deoxyribonucleases adherent cells receptor-mediated luminescent secretory alkaline phosphatases SEAP signals transductions cascades cultures mediums luminescence-based high throughput screening HTS screens agonists reverse reporter assay
• Product Overview:
  The Orexin 1 Receptor (OX1R) may be an important therapeutic target for treatment of sleep disorders, obesity, emotional stress, and addiction. Cayman’s OX1R Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for OX1R, a recombinant G-protein, and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (OX1R Reverse Transfection Strip Plate). Cells grown on the transfection complex will express OX1R at the cell surface and the recombinant G-protein inside the cell. Binding of agonists to OX1R initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture medium. Aliquots of culture medium are removed at time intervals beginning at about 16 hours and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is easy to use and can be easily adapted to high throughput screening for therapeutic compounds regulating activation of OX1R. A known OX1R agonist, Orexin A, is included in the kit for use as a positive control. The kit provides sufficient reagent to measure SEAP activity at three time points, using the black plates provided.