Nrf2 Transcription Factor Assay Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • assays kits measuring measurement TFs factors NF-E2-related 2 Nuclear (erythroid-derived 2)-like 2 NFE2L2 E2 related leucines zippers types zipper-types transcriptions ubiquitously expressed expressions kidneys muscles lungs livers redox homeostasis oxidations reductions interactions cysteines cysteine-rich proteins Kelch like Kelch-like ECH-associated ECH associated 1 Keap1 resting cells complexes targets targeted targeting degrades degradations proteasomes oxidatives stresses undergoes conformational changes phosphoryateds phosphorylations releases releasing Nrf2/Keap1 translocations nucleus nuclei induced inductions genes encodes encoding cytoprotections cytoprotectives antioxidatives antioxidants enzymes activates activations defenses systems cellular molecules molecular damages cancers developments diseases diabetes neurodegenerative neurodegenerations diets dietary factors curcumin downstream lowers lowering inflammations inhibitors inhibits inhibitions carcinogenesis protections xenobiotics drugs interventions disorders nonradioactives non-radioactives non radioactives methods specifics DNA deoxyribonucleic acids binds bindings activity activities nuclear extracts double stranded dsDNA response elements primary antibody antibodies secondary 1 2 HRP horseradish peroxidases colorimetric ELISA EMSA enzyme-linked immunosorbents assays immunoassays radioactives electrophoretics mobility shift genes regulation
• Product Overview:
  Nuclear factor E2-related factor 2 (Nrf2) is a transcription factor that plays a key role in maintaining redox homeostasis via its interaction with a cysteine-rich protein Kelch-like ECH-associated protein 1 (Keap1). In resting cells, Nrf2 and Keap1 form a tight complex, which is targeted for degradation by proteasomes. Under oxidative stress, Nrf2 is released from the Nrf2/Keap1 complex and translocates to the nucleus where it is able to induce the expression of a battery of genes encoding diverse cytoprotective proteins, including antioxidative enzymes, anti-inflammatory mediators, and proteasomes. Cayman’s Nrf2 Transcription Factor Assay is a non-radioactive, colorimetric method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double stranded DNA (dsDNA) sequence containing the Nrf2 response element is immobilized onto the wells of a 96-well plate. Nrf2 contained in nuclear extract samples will bind specifically to the Nrf2 response element and can be detected by addition of a specific Nrf2 antibody and a secondary antibody conjugated to HRP.