NETosis Assay Kit

NETosis Assay Kit

CAT N°: 601010
Price:

544.00 462.40

Cayman’s NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman’s NETosis Assay Kit does not depend upon the DNA component of NETs, as DNA release can occur independently of NETosis. In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore (both included). As shown in Figure 1, unbound neutrophil elastase is washed away following NET generation. Following digestion of NET DNA by S7 nuclease, the supernatant containing neutrophil elastase is added to a substrate, which is selectively cleaved by neutrophil elastase to yield a 4-nitroaniline product that absorbs light at 405 nm. A sufficient amount of each reagent is provided to test and analyze up to 80 individual samples or 24 samples in duplicate.

Territorial Availability: Available through Bertin Technologies only in France

  • Correlated keywords
    • Neutrophil Extracellular Trap kit cell assay measuring measurement measure cell-based CBA neutrophil role engulfment destruction invasive microbe microorganism immune immunity system disease absence dysfunction antimicrobial response trap NET DNA deoxyribonuclease histone macromolecule bacterial plasma DNAse macrophage scavenger receptor-mediated phagocytosis NET/neutrophil complexe component myeloperoxidase MPO elastase NEs lactotransferrin peptide fluorescent fluorescence DNA-intercalating intercalating intercalate dye generation cellular injury injuries necrosis enzyme enzymatic S7 nuclease PMA ionophore A23187 NET-associated associated substrate cleaved cleavages formation NETosis
  • Product Overview:
    Cayman’s NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman’s NETosis Assay Kit does not depend upon the DNA component of NETs, as DNA release can occur independently of NETosis. In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore (both included). As shown in Figure 1, unbound neutrophil elastase is washed away following NET generation. Following digestion of NET DNA by S7 nuclease, the supernatant containing neutrophil elastase is added to a substrate, which is selectively cleaved by neutrophil elastase to yield a 4-nitroaniline product that absorbs light at 405 nm. A sufficient amount of each reagent is provided to test and analyze up to 80 individual samples or 24 samples in duplicate.

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