MitoCheck^® Complex II/III Activity Assay Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • kits assays measures measurements measuring 3 three CoQ coenzymes Q10 cytochromes c oxidoreductases proteins essential oxidatives phosphorylates phosphorylations gatekeeper respirations reactive oxygens species ROSs acceptor electrons e- complexes 1 2 I II one two QH2 ubiquinol ubiquinone carrier bound binds redox reactions reductions oxidations Q-cycles Q cycles Reiske irons sulfurs iron-sulfur Fe-S 2Fe-2S 4Fe-4S 3Fe-4S clusters C1 translocates translocations 4H+ O2- coupled mechanisms inhibits inhibitors inhibitions excess isolates isolations rotenone potassium cyanides KCN
• Product Overview:
  Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration and as a major source of reactive oxygen species III. It accepts electrons from complexes I and II in the form of QH2, the reduced form of the electron carrier ubiquinone. Once bound to complex III, QH2 undergoes a series of redox reactions, known as the Q-cycle. During the Q-cycle, electrons are passed from QH2 onto cytochrome c via the Rieske iron-sulfur protein and cytochrome c1 resulting in the translocation of 4H+ and the generation of 02•-. Cayman’s MitoCheck® Complex II/III Activity Assay measures the reduction of excess cytochrome c (550 nm absorbance) as catalyzed by complex III. This assay is coupled to succinate co-enzyme Q oxidoreductase (complex II) for the generation of QH2. Due to the dependence on complex II activity, a counter-screen for complex II activity (Item No. 700940) should be performed in order to truly measure complex III activity. For the use of this kit with other types of tissue mitochondria, please see Cayman’s MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010).