Melanocortin-4 Receptor Reporter Assay Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • MC-4R melanocortins petides hormones proopiomelanocortins pituitary CNS central nervous systems skin placenta tissues MCR receptors G protein-coupled GPCRs cAMP Adenosine 3′,5′ cyclic mononucleotides messenger signal transduction pathways melanocortin-3 hypothalmus homeostasis fat lean mass hyperphagia MC4R melanocortin-4 adiposity MC3R-knockout mice mouse murine fatty acids oxidations blood pressure hypertension obesity glucose metabolisms insulin insensitivity agonists ligands sexual function antagonists fever suppression inflammatory mediators in vivo Surface transfection and expression protocols solid phase transient transfection technology originus patents USPT US6897067 US6902933 US7056741 US 6897067 6902933 7056741 DNA deoxyribonucleases adherent cells receptor-mediated endocytosis solution-phase co-transfection plasmids secretory alkalines phosphatases substrates SEAP proteins lipids signal transduction luminescence-based cell-based assays kits CBAs high-throughput screening a-MSH alpha-MSH ?-MSH reverse reporter
• Product Overview:
  The melanocortin-4 receptor (MC4R) has important roles in weight regulation, sexual function, and inflammation. Mice deficient in MC4R have increased lipid deposition associated with elevated adiposity, while mutations in MC4R in humans are associated with early onset or severe obesity. This assay consists of a 96-well plate coated with a transfection complex containing DNA constructs for MC4R and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (MC4R Reverse Transfection Strip Plate). Cells grown on the transfection complex will express MC4R at the cell surface. Binding of agonists to MC4R initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit.