L-Selectin/CD62L Extracellular Domain (mouse, recombinant)

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • LECAM-1 CD-62L 62 Ly22 LAM-1 U-937 PSGL1 Gly-CAM-1 GlyCAM1 Trp-39 Ly-22 Ly22 lymph node homing receptor SELL
• Product Overview:
  L-Selectin, also known as CD62L, is a glycoprotein and cell adhesion molecule that is encoded by the SELL gene in humans.{60879,60892} It is composed of an N-terminal calcium-dependent lectin domain that recognizes glycoproteins, an EGF-like domain, two consensus repeats, a transmembrane domain, and an intracellular C-terminal tail. L-selectin is expressed in leukocytes and localizes to the plasma membrane.{60893} It also exists as a soluble form that results from alternative splicing of SELL pre-mRNA or by ectodomain shedding via proteolytic cleavage.{60892} L-selectin facilitates leukocyte rolling on activated vascular endothelium and lymphocyte homing to high endothelial venules of lymph nodes.{60894} It binds to several glycoproteins containing the sulfated sialyl Lewis X (6-sulfo-sLeX) epitope, including P-selectin glycoprotein ligand-1 (PSGL-1) and glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1).{60879} Sell-/- mice exhibit a reduction in the lymphocyte count in peripheral lymph nodes, as well as a decrease in neutrophil recruitment in a model of peritonitis induced by thioglycolate.{60894} Serum levels of soluble L-selectin are lower in patients with sepsis and associated with higher mortality.{60895} Cayman’s L-Selectin/CD62L Extracellular Domain (mouse, recombinant) protein can be used for cell-based assays. This protein is a disulfide-linked homodimer. The reduced monomer, composed of L-selectin (amino acids 1-332) fused to His-tagged human IgG1 at its C-terminus, consists of 542 amino acids, has a calculated molecular weight of 61 kDa, and a predicted N-terminus of Trp39 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 100-110 kDa by SDS-PAGE under reducing conditions.