His-tag Recombinant Monoclonal Antibody

His-tag Recombinant Monoclonal Antibody

CAT N°: 35730
Price:

474.00 402.90

His-tag Recombinant Monoclonal Antibody is a probe for the immunochemical detection of histidine tags on recombinant proteins. Recombinant proteins are commonly labeled with affinity tags, such as six histidine residues (6x-His), to facilitate both their detection and purification.{59520} Poly-His tags are commonly utilized because of their small size, low potential to interfere in protein folding or activity, weak immunogenicity, and high affinity for transition metal ion matrices, such as Ni2+, used in immobilized metal-affinity chromatography (IMAC) for protein purification.{59521,59520} Cayman’s His-tag Recombinant Monoclonal Antibody was produced as a recombinant protein using an engineered construct based on a monoclonal anti-His antibody. This antibody can be used for ELISA and Western blot (WB) applications. It recognizes proteins containing 6x-His tags fused to either the N- or C-terminus and is recognized by anti-mouse IgG secondary antibodies.

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