Glycolysis Cell-<wbr/>Based Assay Kit

Glycolysis Cell-Based Assay Kit

CAT N°: 600450
Price:

439.00 373.15

Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between 490 and 520 nm. The quantity of formazan produced is proportional to the quantity of lactate in the culture medium, and is thus an indirect measurement of glycolysis. This assay can be adapted to high-throughput screening to examine glycolytic regulators in a cell-based system. In addition, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers.

Territorial Availability: Available through Bertin Technologies only in France

  • Correlated keywords
    • CBAs assays kits measures colorimetric colorimetric-based L-lactate culture media mediums cellular metabolic pathways glucose metabolisms cells ATP adenosines triphosphates oxygen exercise erythrocytes mitochondria glucoeogenesis intracellular molecules amino acids insulin glycolytic neurons aerobic energy muscles anaerobic cancers lactate pyruvate tricarboxylic acids cycles oxidizes carbon dioxide CO2 water H2O tumor aggressiveness embryonic stem activity hypoxia-inducible transcriptional factors HIF-1 extracellular inhibition inhibits inhibitors chemotherapeutic strategies strategy metabolomic drugs discovery anticancer
  • Product Overview:
    Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between 490 and 520 nm. The quantity of formazan produced is proportional to the quantity of lactate in the culture medium, and is thus an indirect measurement of glycolysis. This assay can be adapted to high-throughput screening to examine glycolytic regulators in a cell-based system. In addition, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers.

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