DNA Methylation ELISA Kit

DNA Methylation ELISA Kit

CAT N°: 589324
Price:

From 685.00 582.25

DNA methylation is an important epigenetic process regulating gene expression. Methylation occurs on carbon 5 of 2′-deoxy-cytidine yielding the modified base 5-methyl-2′-deoxy cytidine. The methylation pattern of cells is tightly regulated during development with the methylation profile being transmitted from parent to daughter cells during cell division. Methylation results in long-term silencing of genes, while unmethylated regions of DNA can be actively transcribed. Global changes in methylation can be quantified by measuring plasma or urinary levels of 5-methyl-2′-deoxy cytidine. These changes in methylation can provide valuable information about cancer status of an individual. For example, patients with leukemia excrete significantly elevated levels of 5-methyl-2′-deoxy cytidine compared to healthy individuals. Global methylation within tissues can be measured in a similar manner, allowing study of tissue-specific changes that occur as a result of differentiation, aging, or carcinogenesis. Cayman’s DNA Methylation ELISA Kit is a competitive assay that can be used for the quantification of 5-methyl-2′-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices. The ELISA typically displays IC50 (50% B/B0) and IC80 (80% B/B0) values of approximately 12 and 3 ng/ml, respectively.

Territorial Availability: Available through Bertin Technologies only in France

  • Correlated keywords
    • solid plates DNA deoxyribonucleic acids CpG islands dinucleotides genes genomes cancers genomic hypomethylation hypermethylation tumors suppressors malignancy carcinogenesis plasma blood urine leukemia tissues differentiation aging methyltransferases DNMTs DNMT1 DNMT3a DNMT3b nucleosides analogs azacitidine decitabine zebularine drugs suppression competitive assays EIAs ELISAs culture supernatants sample matrices
  • Product Overview:
    DNA methylation is an important epigenetic process regulating gene expression. Methylation occurs on carbon 5 of 2′-deoxy-cytidine yielding the modified base 5-methyl-2′-deoxy cytidine. The methylation pattern of cells is tightly regulated during development with the methylation profile being transmitted from parent to daughter cells during cell division. Methylation results in long-term silencing of genes, while unmethylated regions of DNA can be actively transcribed. Global changes in methylation can be quantified by measuring plasma or urinary levels of 5-methyl-2′-deoxy cytidine. These changes in methylation can provide valuable information about cancer status of an individual. For example, patients with leukemia excrete significantly elevated levels of 5-methyl-2′-deoxy cytidine compared to healthy individuals. Global methylation within tissues can be measured in a similar manner, allowing study of tissue-specific changes that occur as a result of differentiation, aging, or carcinogenesis. Cayman’s DNA Methylation ELISA Kit is a competitive assay that can be used for the quantification of 5-methyl-2′-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices. The ELISA typically displays IC50 (50% B/B0) and IC80 (80% B/B0) values of approximately 12 and 3 ng/ml, respectively.

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