CYP1A1/2 Induction Reporter Assay Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • kits assays measures measurements cytochromes P450s CYP450 CYPs heme-thiolates monoxygenases metabolizes endogenous compounds xenobiotics pollutants environmentals drugs enzymes metabolisms medications induction pharmacokinetics drug-drug interactions co-administered therapeutics CYP1A2 CYP2C19 CYP2C9 CYP2D6 CYP3A4 CYP1A1 CYP1A2 detoxification metabolic polycyclic aromatic hydrocarbons cigarette smoke combustion arylamines heterocyclic amines char-broiled meats intermediates mutagenics carcinogenics inhibits toxicity low efficacy development primary humans hepatocytes livers cells tissues hepatocyte-like CBAs cell-based assays kits measures measurements cultured lines transfected genes promoter/enhancer sequences high-throughput assessments Surface transfection Expression protocols solid phases transient originus, Inc. incorporated University of Michigan U M US patents USPT 6897067 6902933 7056741 complexes DNA deoxyribonucleases proteins adherent endocytosis solution-phase plasmids secreted alkaline phosphatases SEAP inducer-activated transcription factors TFs signals signaling culture mediums luminescence-based 3-MC 3MC reverse reporter assay transfection
• Product Overview:
  Early screening of a drug candidate’s potential to induce or inhibit CYP1A1/2 is a critical step in the drug development process. Cayman’s CYP1A1/2 Induction Reporter Assay Kit is a novel method to assess CYP1A2 induction. Our Reverse Transfection Reporter Assays have overcome many of the disadvantages of other transfection approaches. Cayman’s CYP1A1/2 Induction Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing a Secreted Alkaline Phosphatase (SEAP) reporter construct regulated by the human CYP1A1/2 gene promoter. Cells grown on the CYP1A1/2 Reverse Transfection Strip Plate will introduce the reporter gene into the nucleus. Binding of inducer-activated endogenous transcription factors to the CYP1A1/2 promoter initiates a signal resulting in expression of SEAP which is secreted into the cell culture medium. Aliquots of medium are removed at time intervals beginning at about 24 hours and SEAP activity is measured simply by adding a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high-throughput screening for potential CYP1A1 and CYP1A2 inducers.