€804.00 €683.40
5-Hydroxymethylcytosine (5-hmC) is an epigenetic modification formed from the oxidation of 5-methylcytosine by Tet dioxygenases.{17171} 5-hmC is primarily a stable DNA modification, but it can be oxidized by Tet enzymes and its products further modified to generate nonmethylated cytosine, indicating a role as an intermediate in DNA demethylation as well.{49667,29364,49668} It is associated with actively transcribed genes and recognized by a variety of proteins, including proteins involved in DNA repair and DNA metabolic processes.{49667,49668} 5-hmC has been found in all mammalian tissues but levels are higher in the brain relative to other tissues.{49669} The percentage of genomic 5-hmC in mouse cerebellum increases during postnatal development until adulthood, and genes acquiring intragenic 5-hmC are enriched in pathways associated with age-related neurodegenerative disease pathways in adult mice.{20327} In contrast, 5-hmC levels are reduced by up to 8-fold in cancer tissues.{49667,49670} Cayman’s 5-Hydroxymethylcytosine Polyclonal Antibody can be used for dot blot, ELISA, hydroxymethylated DNA immunoprecipitation (hMeDIP), immunocytochemistry (ICC), and immunohistochemistry (IHC) applications.
Territorial Availability: Available through Bertin Technologies only in France
| Size | 50 µg |
|---|---|
| Shipping | dry ice |
| Molecular weight | 0 |
| Host | Rabbit |
| Antigen | BSA-conjugated 5-hydroxy methylcytosine |
| Clone | RM236 |
| Isotype | IgG |
| Application(s) | Dot blot, ELISA, hMeDIP, ICC, IHC |
| Formulation | 50 µg of protein A affinity-purified antibody from an animal origin–free culture supernatant from an animal origin–free culture supernatant |
| Custom code | 3822.19 |
| UNSPSC code | 12352100 |
Cayman Chemical’s mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.
Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.
Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.
Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.
Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009
Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.
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