AMPK Complex (?1, ?1, and ?1 subunits; human, recombinant)

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • AA MGC 33776 57364 PRKAA 17785 PRKAB 8666 PRKAG 1 2 3 FA
• Product Overview:
  AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase.{57355} It is composed of an ? subunit, two ? subunits, and two ? subunits. The ? and ? subunits each have two isoforms, designated as ?1, ?2, ?1, and ?2, which differ at residues affected by post-translational modifications, and the ? subunit has ?1, ?2, and ?3 isoforms. The diversity of isoforms that can comprise the AMPK complex impart differing functions and subcellular localization.{57355,57356} AMPK is ubiquitously expressed in eukaryotes, and AMPK complexes containing an ?1 subunit are localized to the cytoplasm.{57356} AMPK is activated by phosphorylation of the ? subunit by kinases and/or by AMP or ADP binding to the ? subunit, which occurs under low energy conditions with high ratios of AMP/ADP to ATP, or an increase in intracellular calcium levels.{57355,57357} In this way, AMPK acts as a regulator of cellular energy homeostasis. It can also be activated under conditions of oxidative stress, where it functions as a redox sensor.{57356} Its activity is decreased under high energy conditions where ATP, glucose, fatty acids, and amino acids are plentiful.{57355} AMPK is involved via its downstream signaling pathways in lipid and glucose metabolism, protein synthesis, autophagy, and redox regulation, as well as inhibition of inflammatory processes.{57355,57357} AMPK activation in vivo reduces adiposity in mice, as well as inhibits proliferation of cancer cells in vitro and reduces tumor growth in mouse xenograft models.{57356} Cayman’s AMPK ?1, ?1, and ?1 Subunits (human, recombinant) protein can be used for enzyme activity assay applications. This protein consists of 1,160 amino acids and has a calculated molecular mass of approximately 146 kDa.