AMP<sup>+</sup> MaxSpec<sup>®</sup> Kit

AMP+ MaxSpec® Kit

CAT N°: 710000
Price:

From 97.00 82.45

Many lipids have a free carboxylic acid group that typically requires a weak acid mobile phase to ensure retention by HPLC. Unfortunately, the presence of the acid in the mobile phase also suppresses anion formation and often leads to poor electrospray ionization and poor MS sensitivity. Cayman’s AMP+ MaxSpec® Kit contains a positively charged reagent (AMP+) that can be coupled quantitatively to free carboxylic acids through an amide bond linkage. The resulting derivatized product thus contains a positively charged group that shows improved electrospray ionization and consequently improved MS sensitivity. Cayman’s derivatization reagent is stable to MS/MS fragmentation and thus isobaric derivatives undergo compound-specific fragmentation to give unique fragment ions. Limitations: This kit, when used per the instructions outlined in the booklet, produces derivatized stearic acid and palmitic acid interference peaks. Due to these interferences, this kit should not be used for the detection or identification of these two acids in samples.{60074,60075}

Territorial Availability: Available through Bertin Technologies only in France

  • Correlated keywords
    • MS spec kits assays measures measurements N-(4-aminomethylphenyl)pyridinium AMPP salts quaternary ammonium reagents eicosanoids sensitivity sensitivities Michael H. Frantisek Turecek James Bollinger US 2011/0294153 USPT A1 13/078,652 carboxylic acids derivatives pyridiniums C12H13N2+ charged chemicals free amides compound-specific fragmentations MS/MS derivative-specific AMP-derivatized AMP+-derivatized
  • Product Overview:
    Many lipids have a free carboxylic acid group that typically requires a weak acid mobile phase to ensure retention by HPLC. Unfortunately, the presence of the acid in the mobile phase also suppresses anion formation and often leads to poor electrospray ionization and poor MS sensitivity. Cayman’s AMP+ MaxSpec® Kit contains a positively charged reagent (AMP+) that can be coupled quantitatively to free carboxylic acids through an amide bond linkage. The resulting derivatized product thus contains a positively charged group that shows improved electrospray ionization and consequently improved MS sensitivity. Cayman’s derivatization reagent is stable to MS/MS fragmentation and thus isobaric derivatives undergo compound-specific fragmentation to give unique fragment ions. Limitations: This kit, when used per the instructions outlined in the booklet, produces derivatized stearic acid and palmitic acid interference peaks. Due to these interferences, this kit should not be used for the detection or identification of these two acids in samples.{60074,60075}

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