Vitamin D ELISA Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • kits assays measures measurements EIAs ELISAs enzymes immunoassays immunosorbents vitamins functions regulates intestines intestinal absorptions calcium Ca2+ phosphorus P bones growths immune immunity cells communications extra-skeletons extra skeletal skeletons effects cardiovascular endocrines systems central nervous CNS functional synthesis syntheses skins cholesterols by-products by products 7-dehydrocholesterol 7 dehydro UV ultaviolet lights D3 3 cholecalciferol bloodstreams binding proteins livers 25-hydroxy 25 hydroxy 25-OH OH 25(OH)D3 calcidiol converts conversions converted kidneys 1,25-dihydroxycholecalciferol 1,25 dihydroxy 1,25-(OH)2 (OH)2 1,25(OH)2D3 calcitriol active forms short-lived short lived metabolized metabolizes metabolizing deactivated forms 24,25 dihydroxy 24,25-(OH)2 24,25(OH)2D3 1,24,25 trihydroxy 1,24,25-(OH)3 (OH)3 1,24,25(OH)3D3 diets nutritions dietary supplements activations yeasts fungus fungi ergocalciferol produces biosynthetic routes biologically active plasmas serums detects detections metabolisms metabolically stable displaced displacements humans competitives quantifications quantifying quantifies determination measurements
• Product Overview:
  The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D3. Vitamin D3 is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it is converted to 25-hydroxy vitamin D3 (25(OH)D3). This product is then transported to the kidney where it is further converted to 1,25-dihydroxycholecalciferol (1,25(OH)2D3), the biologically active form. Active 1,25(OH)2D3 is very short-lived and is rapidly metabolized to its deactivated forms. Vitamin D Immunoassay: Accurate Vitamin D measurement in plasma or serum Cayman’s Vitamin D EIA Kit is a competitive assay that can be used for Vitamin D quantification in plasma or serum. Because of the short half-life of the biologically active 1,25(OH)D form, this Vitamin D assay primarily detects the more metabolically stable forms, 25(OH)D3 and 25(OH)D2. Accurate detection of these forms requires that they be displaced from the vitamin D binding protein prior to measurement. A simple, acetone-based purification protocol is included as the initial procedure of the Vitamin D assay kit for this purpose.