€1,183.00 €1,005.55
Diphtheria toxin is a toxin produced by C. diphtheriae that catalyzes the ADP-ribosylation and inactivation of translation elongation factor 2 (EF-2). It is synthesized and excreted as a proenzyme and must undergo two covalent alterations in structure in order to be active. First, mild proteolysis results in the formation of “nicked toxin”, which is enzymatically inactive and consists of two major fragments, A and B. Reduction of the nicked toxin with thiols releases the N-terminal A fragment, which is enzymatically active. The C-terminal B fragment binds to specific host receptors and translocates the catalytic domain into the cell.{31323} After binding to the cell receptor, the diphtheria toxin is taken up by endocytosis, the pH of the endocytic vesicle drops, and the translocation region of the toxin helps guide the catalytic domain into the host cytoplasm where it is released.{31324} Within the cytoplasm, the diphtheria toxin catalytic domain ADP ribosylates EF-2, terminating protein synthesis and causing cell death.{31322}
Territorial Availability: Available through Bertin Technologies only in France
| Size | 1 mg |
|---|---|
| Shipping | wet ice |
| Molecular weight | 0 |
| Formulation | Each vial, when reconstituted to 0.5 ml with water, contains 1 mg of unnicked diphtheria toxin in 0.01 M Tris and 0.001 M EDTA at pH 7.5 |
| Purity | ≥90% estimated by SDS-PAGE |
| Custom code | 3504.00 |
| UNSPSC code | 12352204 |
Cayman Chemical’s mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.
Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.
Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.
Our organic and analytical chemists specialize in the rapid development of manufacturing processes and analytical methods to carry out clinical and commercial GMP-API production. Pre-clinical drug discovery efforts are currently underway in the areas of bone restoration and repair, muscular dystrophy, oncology, and inflammation. A separate group of Ph.D.-level scientists are dedicated to offering Hit-to-Lead Discovery and Profiling Services for epigenetic targets. Our knowledgeable chemists can be contracted to perform complete sample analysis for analytes measured by the majority of our assays. We also offer a wide range of analytical services using LC-MS/MS, HPLC, GC, and many other techniques.
Accreditations
ISO/IEC 17025:2005
ISO Guide 34:2009
Cayman is a leader in the field of emerging drugs of abuse, providing high-purity Schedule I-V Controlled Substances to federally-licensed laboratories and qualified academic research institutions for forensic analyses. We are certified by ACLASS Accreditation Services with dual accreditation to ISO/IEC 17025:2005 and ISO Guide 34:2009.
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