Coelenter<wbr/>azine 400a

Coelenterazine 400a

CAT N°: 16157
Price:

From 140.00 119.00

Coelenterazine 400a is a bisdeoxy derivative of coelenterazine (Item No. 16123) that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).{26297,26291} It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h (Item No. 16894), which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.{26296} Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.{26291,26296} BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.{26297,26291,26294}

Territorial Availability: Available through Bertin Technologies only in France

  • Synonyms
    • 6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one
  • Correlated keywords
    • fluorescent probes intracellular signaling coelenterazine hh bisdeoxycoelenterazine BDC bisdeoxy derivatives Renilla luciferase BRET BRET1 BRET2 proteins protein-protein interactions Rluc GFP receptors luciferin substrates photoproteins oxidation energy dideoxycoelenterazine Deep Blue C DeepBlueC™
  • Product Overview:
    Coelenterazine 400a is a bisdeoxy derivative of coelenterazine (Item No. 16123) that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).{26297,26291} It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h (Item No. 16894), which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.{26296} Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.{26291,26296} BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.{26297,26291,26294}

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