Xanthine Oxidase Fluorometric Assay Kit

Xanthine Oxidase Fluorometric Assay Kit

CAT N°: 10010895
Price:

419.00 356.15

Xanthine oxidase (XO) catalyzes the hydroxylation of hypoxanthine to xanthine and then further catalyzes the oxidation of xanthine to uric acid. When oxidizing NADH, XO generates superoxide, a powerful reactive oxygen species. Excessive amounts of superoxide can lead to cell degeneration and death. XO is found predominantly in the liver and intestine. However, levels of circulating XO can increase dramatically with disease. Cayman’s Xanthine Oxidase Assay Kit provides a simple and accurate method for quantifying xanthine oxidase activity. The assay is based on a multistep enzymatic reaction whose end product resorufin, a highly fluorescent compound, can be easily analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm.

Territorial Availability: Available through Bertin Technologies only in France

  • Correlated keywords
    • assays kits plasma serum tissues homogenates oxidoreductases molybdoflavoenzymes human purine catabolism catalyzing catalyzes hydroxylation hypoxanthine uric acids NADH superoxides reactive oxygen species ROS hydrogen peroxides H2O2 inschemia-reperfusion injury molecular cells death degeneration apoptosis mammalian livers intestines diseases horseradish HRP ADHP fluorescence fluorescent resorufin excitation emission wavelengths special:quarterly specialdiscount:50 specialstart:2007-03-01 specialend:2008-05-31 fluorometric
  • Product Overview:
    Xanthine oxidase (XO) catalyzes the hydroxylation of hypoxanthine to xanthine and then further catalyzes the oxidation of xanthine to uric acid. When oxidizing NADH, XO generates superoxide, a powerful reactive oxygen species. Excessive amounts of superoxide can lead to cell degeneration and death. XO is found predominantly in the liver and intestine. However, levels of circulating XO can increase dramatically with disease. Cayman’s Xanthine Oxidase Assay Kit provides a simple and accurate method for quantifying xanthine oxidase activity. The assay is based on a multistep enzymatic reaction whose end product resorufin, a highly fluorescent compound, can be easily analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm.

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