SREBP-1 Transcription Factor Assay Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • tf atherosclerosis SREBPs sterols regulatory element binding proteins factors basic helix-loop-helix-leucine zipper family expression regulate synthesis regulation uptake cholesterol fatty acids triglycerides phospholipids adipocyte differentiation insulin-dependent genes SREBP-1a membrane-bound rough ER endoplasmic reticulum cleavage activating SREBP-Scap Golgi proteolytic two-step cleavage S1P S2P NH2 -terminal active domain nuclear localization signal importin nucleus upregulating genes fatty acids lipogenic enzymes acetyl CoA coenzymes A carboxylases synthases long chain elongases glucose glucokinases glycolysis diseases obesity diabetes mellitus insulin resistance non-alcoholic liver non-radioactive sensitve sensitivity detects detecting double stranded dsDNA extracts ELISA EMSA humans rats mouse mice murine SREBP1C SREBP 1c assays
• Product Overview:
  Three known isoforms of SREBP (sterol regulatory element binding protein) transcription factors have been characterized: SREBP-1a, SREBP-1c, and SREBP-2. SREBP-1c acts primarily to activate genes required for fatty acid synthesis, such as acetyl-CoA carboxylase, fatty acid synthase, and long chain fatty acid elongase. In addition, SREBP-1c may also contribute to the regulation of glucose uptake and synthesis through induction of glucokinase. SREBP-1c has important clinical implications in the treatment of many diseases including obesity, diabetes mellitus, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Cayman’s SREBP-1 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity. SREBP-1 contained in nuclear extracts and whole cell lysates binds to a dsDNA SREBP response element immobilized to the wells of a 96-well plate. SREBP-1 is detected by addition of a specific primary antibody directed against SREBP-1. A secondary antibody conjugated to HRP is used to provide a sensitive colorimetric readout at 450 nm.