MitoCheck^® Complex I Activity Assay Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • kits assays measures measurements 1 one nicotinamides adenines dinucleotides reduced NADH oxidases Co-enzymes Q oxidases/Co-enzymes CoQ10 reductases e- electrons mitochondrial mitochondrias transports chains ETCs catalyzes two 2 II oxidations reduces reductions ubiquinones ubiquinols QH2 terminal acceptors oxygens O2 translocations translocalizations 4 four protons H+ matrix matrices intermembrane spaces chemiosmotic gradients oxidative phosphorylations phosphorylates inhibits inhibitors inhibitions dysfunctions produces productions reactive species ROSs neurological brains disorders Parkinson’s Parkinsons diseases Down’s Downs Leigh’s Leighs syndromes indicates indicators defects toxicity toxicities effects rotenone piericidin isolates isolations
• Product Overview:
  Complex I (NADH dehydrogenase/NADH:ubiquinone oxidoreductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the reduction of ubiquinone (Q) to form ubiquinol (QH2), and ultimately the reduction of the terminal electron acceptor, O2. During the passage of electrons from NADH to Q, the translocation of four protons (H+) from the mitochondrial matrix to the intermembrane space occurs, contributing to the chemiosmotic proton gradient, which is required for oxidative phosphorylation. Cayman’s MitoCheck® Complex I Activity Assay allows for the activity of complex I to be determined without the need to isolate mitochondria or pre-incubate with antibodies. The rate of NADH oxidation is measured by a decrease in absorbance at 340 nm and is proportional to the activity of complex I. For the use of this kit with other types of tissue mitochondria, please see Cayman’s MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010).