Aldehyde Dehydrogenase Activity Assay Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • kits assays measures measurements ALDHs enzymes oxidizes oxidations endogenous exogenous aldehydes dehydrogenases carboxylic acids metabolisms amino carbohydrates lipids biogenic amines vitamins steroids humans superfamily isozymes physiological toxicological ALDH1A subfamily embryogenesis developments mediates mediating retinoic signaling signals signalling ALDH2 acetaldehydes cells tissues alcohol ingestions carcinogenesis ALDH1A1 ALDH3A1 lens corneal crystallins cellular defenses mechanisms UV ultraviolet radiations radiation-induced damages ocular cancers stems pharmacologicals inhibits inhibitors inhibitions anticancers anti anti-cancers oxazaphophorines drugs diseases Type II 2 hyperprolinemia Sjogrens Sjogren-Larsson Syndromes Parkinson’s cardiac hearts hyperammonemia fluoroescence-based methods fluorescent homogenates cultures samples purified preparations fluorometrics
• Product Overview:
  Aldehyde dehydrogenases (ALDHs) represent a group of enzymes that oxidize a wide range of endogenous and exogenous aldehydes to their corresponding carboxylic acids, which affect both developmental and toxicological functions. Pharmacological inhibitors or activators of ALDH activity have been developed to examine the metabolism of alcohol as well as a number of pathological conditions, including cancer, Type II hyperprolinemia, Sjögren-Larsson Syndrome, Parkinson’s Disease, Cardiac Disease, and hyperammonemia. Cayman’s Aldehyde Dehydrogenase Assay provides a fluorescence-based method for detecting ALDH activity in tissue homogenates, cell culture samples, and purified ALDH preparations. In the assay, ALDH catalyzes the oxidation of acetaldehyde to acetic acid, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorometric developer to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.