β-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit

β-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit

CAT N°: 700740
Price:

516.00 438.60

Cayman’s ?-HB (Ketone Body) Fluorometric Assay Kit provides a simple, reproducible, and sensitive tool for assaying ?-HB from plasma, serum, urine, tissue homogenates, and cell lysates. The method for ?-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme 3-hydroxybutyrate dehydrogenase.16 Concomitant with this oxidation, the cofactor NAD+ is reduced to NADH. NADH reacts with the fluorometric developer to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The fluorescence is directly proportional to the ?-HB concentration.

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