EP₂ Receptor (rat) Reporter Assay Kit

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • prostaglandins E2 PGE2 prostanoids G protein-coupled receptors GPCRs EP1 EP3 EP4 signal transduction tissues regulation cells mediates cAMP cyclic messenger pathways mucosal protection gastrointestinal secretions motility immunity inflammation mice mouse murine tumors cancers cancer-associated immunodeficiency dendritic-cell differentiation in vitro neuroprotective NMDA toxicity oxygen glucose deprivation agonists antagonists therapeutics Surface transfection and expression protocols solid phase transient transfection technology originus patents USPT US6897067 US6902933 US7056741 US 6897067 6902933 7056741 DNA deoxyribonucleases adherent cells receptor-mediated endocytosis solution-phase co-transfection plasmids secretory alkalines phosphatases substrates SEAP proteins lipids signal transduction luminescence-based cell-based assays kits CBAs high-throughput screening reverse reporter assay
• Product Overview:
  The EP2 receptor is one of four GPCRs that mediate the actions of PGE2. The diverse effects of PGE2 acting via EP2 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman’s EP2 Receptor (rat) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for rat EP2 receptor and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (EP2 Receptor Reverse Transfection Plate). Cells grown on the transfection complex will express EP2 at the cell surface. Binding of agonists to EP2 initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. Aliquots of media are removed at time intervals, beginning at approximately six hours, and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds regulating the activation of EP2. PGE2 is included in the kit for use as a positive control. The kit provides sufficient reagents to measure SEAP activity at three time points using the black plates included.