HIF-1? (human, recombinant; aa 575-826)

Territorial Availability: Available through Bertin Technologies only in France

• Correlated keywords
  • HIF1? PERARNTSIM bHLHPAS HIF1? HIF1 HIF-1-? HIF1? basic helix loop PAS protein MOP1 MOP-1 bHLHe78 bHLHE-78 class E basic PAS domain containing protein 8 Q-16665
• Product Overview:
  Hypoxia-inducible factor-1? (HIF-1?) is a transcription factor subunit that belongs to the basic helix-loop-helix PER-ARNT-SIM (bHLH-PAS) protein family.{11044,46550} It contains bHLH and PAS domains that mediate DNA binding and heterodimerization with the HIF-1? subunit, an oxygen-dependent degradation (ODD) domain that is hydroxylated by prolyl hydroxylase in the presence of oxygen to target HIF-1? for proteasomal degradation, and N- and C-terminal transactivation domains responsible for regulating the expression of HIF-1 target genes.{46550,46551} Under hypoxic conditions, HIF-1? is stabilized, accumulates in the cytoplasm, and is translocated to the nucleus where it forms a heterodimer with HIF-1? and induces the expression of genes involved in maintaining cellular oxygen homeostasis.{11044,46550,10948,12428} It is also involved in angiogenesis, glucose utilization, and pH regulation under hypoxic conditions, including in the tumor microenvironment.{17023} HIF-1? is overexpressed in a variety of cancer cell lines where it promotes survival of cancer cells and increases invasiveness under hypoxic conditions and, in vivo, overexpression is associated with aggressiveness and progression of various cancers and poor disease-free survival.{17023,9833,46552,46553} Homozygous knockout of HIF-1? is embryonic lethal due to disruptions in vascular development but conditional knockout models have demonstrated a role for HIF-1? in inflammation, immunity, and osteogenesis.{17023} Cayman’s HIF-1? (human, recombinant; aa 575-826) protein consists of 259 amino acids and has a calculated molecular weight of 28.4 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 34 kDa.