5-<wbr/>Cyano-<wbr/>2,3-<wbr/>di-<wbr/>(<em>p-<wbr/></em>tolyl)tetrazolium (chloride)

5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride)

CAT N°: 15926
Price:

48.00 40.80

5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms.{25823,25821,25822} This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm).{25822,25820} While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter.{25823,25821} For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable.

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