3-Indoxyl Phosphate (sodium salt)

3-Indoxyl Phosphate (sodium salt)

CAT N°: 33936
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165.00 140.25

3-Indoxyl phosphate is a chromogenic and electrochemical substrate for alkaline phosphatase.{61471,61472} Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity.{61471} 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.{61472} 3-Indoxyl phosphate has been used in ELISAs.

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