13,14-dihydro-15-keto Prostaglandin E₂-d₄ MaxSpec^® Standard

Territorial Availability: Available through Bertin Technologies only in France

• Synonyms
  • 9,15-dioxo-11?-hydroxy-prost-5Z-en-1-oic-3,3,4,4-d4 acid
• Correlated keywords
  • deuterated deuterium quantitative standard mass Max spec spectrometry lipidomics LC-MS LCMS
• Product Overview:
  13,14-dihydro-15-keto PGE2-d4 MaxSpec® Standard is intended for use as an internal standard for the quantification of 13,14-dihydro-15-keto PGE2 (Item No. 14010) by GC- or LC-MS. 13,14-dihydro-15-keto PGE2 is a metabolite of PGE2 (Item No. 14010) and the primary PGE2 metabolite in plasma.{182,2777} It is formed from PGE2 via a 15-keto PGE2 intermediate by 15-oxo-PG ?13 reductase.{2777} Unlike PGE2, 13,14-dihydro-15-keto PGE2 does not bind effectively to the PGE2 receptors EP2 and EP4 expressed in CHO cells (Kis = 12 and 57 µM, respectively) or induce adenylate cyclase activity in the same cells (EC50s = >18 and >38 µM, respectively). Levels of 13,14-dihydro-15-keto PGE2 are increased in the plasma of women in the third trimester of pregnancy and in women during and immediately after labor and delivery.{64194} Levels of 13,14-dihydro-15-keto PGE2 levels are decreased in tumor tissue compared to adjacent non-cancerous tissue isolated from patients with non-small cell lung cancer (NSCLC).{64195} 13,14-dh-15-keto PGE2-d4 MaxSpec® standard is a quantitative grade standard of 13,14-dh-15-keto PGE2-d4 (Item No. 10010606) that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This 13,14-dh-15-keto PGE2-d4 MaxSpec® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.