(±)12(13)-DiHOME MaxSpec^® Standard

Territorial Availability: Available through Bertin Technologies only in France

• Synonyms
  • 12,13-dihydroxy-9Z-octadecenoic acid
• Correlated keywords
  • quantitative standard mass Max spec spectrometry lipidomics LC-MS LCMS P 450 iso leukotoxin toxin Sf 21
• Product Overview:
  (±)12(13)-DiHOME is the diol form of (±)12(13)-EpOME (Item No. 52450), a cytochrome P450-derived epoxide of linoleic acid (Item Nos. 90150 | 90150.1 | 21909) also known as isoleukotoxin.{8529} It is formed from 12(13)-EpOME by soluble epoxide hydrolase (sEH) in neutrophils.{17057} 12(13)-DiHOME is toxic to Sf21 cells expressing sEH and to lacZ-expressing control cells, unlike isoleukotoxin, which is only toxic to cells containing sEH.{8529,17057} Levels of 12(13)-DiHOME are increased in rat spinal cord following burn injury, and it enhances cold tolerance, increases fatty acid uptake into brown adipocytes, and decreases serum triglyceride levels in mice.{35412,34553} Levels are also elevated in bronchoalveolar lavage fluid (BALF) in humans following exposure to biodiesel exhaust and in exhaled breath condensate in patients with allergic asthma following allergen exposure.{42556,42557} Plasma levels of 12(13)-DiHOME are increased immediately following moderate-intensity exercise in mice and humans, an effect that can be prevented by brown adipose tissue removal in the mouse.{35413} (±)12(13)-DiHOME MaxSpec® standard is a quantitative grade standard of (±)12(13)-DiHOME (Item No. 10009832) that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This (±)12(13)-DiHOME MaxSpec® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.